COLIKAT RAPID® is a fast test to simultaneously detect both total coliforms and Escherichia coli in water samples providing the test’s results within 18 hours. COLIKAT RAPID® is suited for the entire water matrix.

• Simultaneous detection and quantification of Escherichia coli and total coliforms for the entire water matrix.
• Sample evaluation after 18 hours.
• Optimized workflow in your laboratory: The sample evaluation takes place after 18 hours before the reception of next days’ samples.
• Enumeration of Escherichia coli and coliform bacteria in water samples acc. to EN ISO 9308-2.

Test Principle

COLIKAT RAPID® uses certain specific characteristics of both Escherichia coli and coliform bacteria for identification of the species. The test contains MUG and ONPG, two common nutritional indicators. Coliform bacteria use their enzyme β-Galactosidase to metabolize ONPG turning the sample yellow. Escherichia coli – other then most coliform bacteria – additionally has the enzyme β-Glucuronidase that enables it to hydrolyze MUG so that the sample additionally shows fluorescence under UV-light.

Most of non-coliform bacteria possess neither the β-Galactosidase nor the β-Glucuronidase. Therefore, they cannot transform MUG and ONPG and the test will not detect them. Yet, some non-coliform bacteria contain the enzymes. COLIKAT RAPID® therefore uses selective supplements that inhibit the growth of those bacteria. The combination of the test’s enzymes and selective supplements prevents some common problems of conventional culture methods such as Chromogen Coliform Agar CCA (EN ISO 9308-1):

• False positive results are prevented (for example for Aeromonas spec.)
• The growth of non-target organism is prohibited. This way non-target organisms cannot affect (or even inhibit) the growth of Escherichia coli and coliform bacteria

• To assist distinguishing positive from negative results by comparing the color of the Compare Kit with the color of the sample
• Available in vessel, 51-well and 97-well format

• For the simultaneous identification and quantification of Escherichia coli and coliform bacteria
• 3-side pre-sealing insures easy filling of the COLIKAT Enumeration Tray
• Easy and fast sealing to separate the cavities with the COLIKAT Seal Applicator
• Available in 51-well and 97-well format

• 125ml and 300ml vessels
• Available with and without sodium thiosulfate
• Available with and without anti-foaming agent

• Compact sealer to seal the COLIKAT Enumeration Tray 51 and 97
• Safe separation of the cavities for valid quantification